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项目作者： not-a-feature

项目描述 ：
A simple ImageJ Macro to count the cells of multiple images.
高级语言： ImageJ Macro
项目地址: git://github.com/not-a-feature/MultiImageCellCounter.git
创建时间： 2021-05-17T12:42:59Z
项目社区：https://github.com/not-a-feature/MultiImageCellCounter
开源协议：GNU General Public License v3.0
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Multi Image Cell Counter (MICC)

MICC is a simple ImageJ - Macro to count cells of multiple images at once.

Requirements

  • ImageJ 1 (may also work on ImageJ 2)

Installation

  • Download the MultiImageCellCounter.ijm (or download the whole project)
  • No installation is required.

Usage

  • Click on Plugins -> Macros -> Run...
  • Set the desired parameters
  • Click on ok

screenshot

Parameters

Path to folder

  • Path to the folder containg the images.
  • Please make sure only images are inside this folder.

Minimum cell size

  • Minimum cell size.
  • The higher, the less artifacts are detected

Minimum cell size

  • Maximum cell size.
  • Set to 0 if no maximum cell size is desired.

Minimum / Maximum cell circularity

  • Roundness of cell

Fill holes

  • Fill the holes of the generated mask. This setting should not have a major impact.

Watershed

  • Watersheds image.
  • Only turn off if the cells are very scattered.

Exclude on edges

  • Excludes cells located at the edge of the image

Channel

  • Select channel of image.
    • Open one of the image-files.
    • If several windows are opening close every window except the desired one.
    • Take a look at the title. E.g.: “123-Alexa555.zvi Ch0”
    • The last number is the channel.
  • Set to -1 if the image has only one channel.

Output files directory

  • If this is set, this programm will save the processed (counted) images to this folder.

License

  1. Copyright (C) 2021 by Jules Kreuer - @not_a_feature
  2. This piece of software is published unter the GNU General Public License v3.0
  3. TLDR:
  5. | Permissions      | Conditions                   | Limitations |
  6. | ---------------- | ---------------------------- | ----------- |
  7. | ✓ Commercial use | Disclose source              | ✕ Liability |
  8. | ✓ Distribution   | License and copyright notice | ✕ Warranty  |
  9. | ✓ Modification   | Same license                 |             |
  10. | ✓ Patent use     | State changes                |             |
  11. | ✓ Private use    |                              |             |

Go to https://github.com/not-a-feature/MultiImageCellCounter/blob/main/LICENSE to see the full version.