ORIGAMI - A Software Suite for Activated Ion Mobility Mass Spectrometry Applied To Multimeric Protein Assemblies

ORIGAMI is a two component software suite for faster acquisition of actived ion mobility mass spectrometry (IM-MS) datasets and analysis of MS and IM-MS data.

The acquisition software (ORIGAMI^MS) works by interfacing MassLynx and Waters Research Enabled Software (WREnS) to carry out a typically tedious task of increasing the collision or cone voltage prior to ion mobility separation. The program works by executing a set of commands that modify various DC potentials in Waters Synapt G2 (and above) family of instruments. There are four acquisition modes available (linear, exponential, Boltzmann and User-defined) which determine the activation ramp parameters.

ORIGAMI^MS requirements:

Installed WREnS on your instrument PC (please contact Waters Corp. for access to WREnS and installation instructions)
Download ORIGAMI^MS and associated WREnS scripts
Read the User Guide for more details or watch the YouTube video
Installation instructions can be found in the User Guide

The analysis software (ORIGAMI^ANALYSE) can import single or multiple IM-MS Waters files (.raw) or a list of text files (.csv, .txt) to visualise and interrogate the ion mobility data. It provides an analysis platform to extract, process, visualise and export MS and IM-MS data to convenient image or text formats. The program was designed to handle activated IM-MS datasets, hence can easily manipulate large number of files to generate CIU or SID fingerprint maps; datasets can be compared and overlayed to produce high-quality images. All of the processed data can be also exported in an interactive format, to give a webpage-like behaviour with utility tools such as zooming, panning, data hovering and much more.

Some ORIGAMI^ANALYSE features:

import single or multiple Waters raw files (requires Driftscope)
import single or multiple text files
batch data extraction from raw files
comparison and overlay of CIU/SID/UVPD datasets
export all data as static image or in an interactive webpage format
create and apply CCS calibration to proteins and small molecules
tools to analyse linear DTIMS datasets

ORIGAMI^ANALYSE requirements:

Driftscope (should be provided with your Waters instrument) - if Driftscope is not present, you will not be able to extract MS or IM-MS data
Read the User Guide
Installation instructions can be found in the User Guide

Authors and contributors:

Lukasz G. Migas (lukasz.migas@manchester.ac.uk) - Software development and data acquisition
Aidan France (aidan.france@manchester.ac.uk) - Data acquisition
Perdita E. Barran (perdita.barran@manchester.ac.uk) - Supervision
Bruno Bellina (bruno.bellina@manchester.ac.uk) - Supervision
PB Research Group - Testing 😄
Other users - bug reports 🐛

New features and bug reports

If you encounter any errors or problems, would like to ask request new features or just have a chat about ORIGAMI or other MS and IM-MS topics, please contact Lukasz Migas (lukasz.migas@manchester.ac.uk).

Citation

If you use either of the components of ORIGAMI, please consider citing it in your work:

L. G. Migas, A. P. France, B. Bellina, P. E. Barran, “ORIGAMI: A software suite for activated ion mobility mass spectrometry (aIM-MS) applied to multimeric protein assemblies”, Int. J. Mass Spectrom., 2017