Statistical analysis of gene expression data with multiple comparison tests in R.
This repo goes through the workflow of differential expression of RNA-Seq (gene expression) data using the DESeq package, starting with raw counts of sequencing reads.
The dataset used is the Pasilla dataset, available from the Pasilla Bioconductor library. It contains RNA-Seq count data for RNAi treated and untreated Drosophila melanogaster cells.
RNASeq.R
RNASeq_with_DNASeq.md goes through the workflow.
A full description of the project and workflow can be found here.