Bismark-MethylDackel DNA methylation quantification pipeline in snakemake.
Bismark-MethylDackel pipeline in snakemake.
This pipeline guarantees reproducible results, which means that it guarantees same output (DNA methylation levels) with same input (Bisulfite sequencing reads). The reproducibility is continuously being tested and the result is shown as a badge above.
$ git clone https://github.com/dohlee/snakemake-bismark-methyldackel.git$ cd snakemake-bismark-methyldackel
Modify the configurations manifest file as you want.
Run the pipeline.
If you already have snakemake installed, it might be useful to just use --use-conda option. Tweak -j parameter according to the number of available cores on your system.
$ snakemake --use-conda -p -j 32
Or you can create separate environment for this pipeline and run it.
$ conda env create -f environment.yaml$ snakemake -p -j 32